Journal: The EMBO Journal

Article Title: NPC1 regulates the distribution of phosphatidylinositol 4‐kinases at Golgi and lysosomal membranes

doi: 10.15252/embj.2020105990

Figure Lengend Snippet:

Article Snippet: AffinityScript QPCR Synthesis Kit , Agilent technologies , Cat#600559.

Techniques: Recombinant, Electron Microscopy, Blocking Assay, Protease Inhibitor, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Sequencing, Software, Imaging

Journal: Viruses

Article Title: Bacteriophage 434 Hex Protein Prevents RecA-Mediated Repressor Autocleavage

doi: 10.3390/v5010111

Figure Lengend Snippet: RT-PCR analysis of total bacterial RNA isolated from MG1655::λ imm434 lysogens. Total RNA was reverse transcribed using Primer 1 (see ). The cDNA was PCR separately amplified with primers 2 + 3 (lane 1) or 1 + 3 (lane 2), and products separated on an agarose gel. The numbers represent sizes of molecular weight standards.

Article Snippet: Synthesis of first-strand cDNA was performed using AffinityScript TM QPCR cDNA Synthesis Kit (Qiagen Valencia, CA, USA).

Techniques: Reverse Transcription Polymerase Chain Reaction, Isolation, Reverse Transcription, Amplification, Agarose Gel Electrophoresis, Molecular Weight

Journal: Materials

Article Title: 3D-Printing of Hierarchically Designed and Osteoconductive Bone Tissue Engineering Scaffolds

doi: 10.3390/ma13081836

Figure Lengend Snippet: Detection of calcium deposition via alizarin staining on undyed scaffold prototype coated with Saos-2 cells after 21 days: ( a ) left scaffold was seeded in RPMI-medium; ( b ) right scaffold was incubated with osteogenic differentiation medium; and ( c ) PCR results for osteogenic differentiation on Day 21. Saos-2 cells seeded on scaffolds were incubated with RPMI medium and osteogenic differentiation medium. Focus was on cDNA for osteogenic proteins: collagen 1 (Col1), alkalic phosphatase (ALP), and osteocalcin (BGLAP). GAPDH was used as internal standard. The 2 −(ΔΔCt) values are presented.

Article Snippet: Contaminating DNA was removed with a RNase-Free DNase Kit (Qiagen, Hilden, Germany) following the manufacturer´s instructions followed by first strand cDNA synthesis using an AffinityScript PCR cDNA Synthesis Kit (Stratagene, La Jolla, USA).

Techniques: Staining, Incubation